Device

Part:BBa_K2088010:Design

Designed by: Shi Kejian   Group: iGEM16_NAU-CHINA   (2016-10-08)


A device that expresses the XylE- catechol 2,3-dioxygenase to degrade the catechol .


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 400
    Illegal NgoMIV site found at 572
    Illegal AgeI site found at 923
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We linked the K316003 with the promoter J23100 and the RBS B0034 ahead.



Source

We use the parts in kit2016 to complete the device.


References